Which line is C located on in alto clef?

In the alto clef, middle C is specifically located on the third line. The alto clef is centered around this line, making it a critical reference point for players, particularly in instruments like the viola, which commonly uses this clef. The notes arranged on the staff lines in ascending order from the bottom are E, G, B, D, and F. Therefore, since the third line represents the note C, this confirms that C is indeed positioned on that line. Understanding the placement of notes in different clefs is essential for reading and performing music accurately.